PTPsigma/PTPRS Active human

半岛bd体育手机客户端 说明

一般描述

Protein tyrosine phosphatase, receptor type S (PTPRS) also known as PTPσ belongs to the subfamily of receptor-type protein tyrosine phosphatases. It is encoded by the gene mapped to human chromosome 19p13.3. PTPRS consists of large ectodomain and a membrane-spanning intracellular domain.
Human PTPRS (GenBank Accession No. BC104812) (amino acids 883-end), with N-terminal GST tag, MW = 98kDa, expressed in aEscherichia coliexpression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

Protein tyrosine phosphatase, receptor type S (PTPRS)/ PTPs localized on the cell surface plays a vital role in cell–cell and cell– extracellular matrix (ECM) interactions, and is implicated in the regulation of axon homeostasis and neuronal development. In addition, PTPRS also has a crucial role in autophagy regulation. PTPRS expressed on plasmacytoid dendritic cells (pDCs) inhibits the production of type I interferon (IFN) in response to viruses. Mutation of both Ptprs and Ptprf (protein tyrosine phosphatase, receptor type F) results in pDC hyperactivation and mild colitis.

单位定义

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
测定	≥70% (SDS-PAGE)
形式	aqueous solution
分子量	98 kDa
包装	pkg of 20 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	>0.02 mg/mL
NCBI登记号	BC104812
UniProt登记号	Q13332
运输	dry ice
储存温度	?70℃
Gene Information	human ... PTPRS(5802)