半岛bd体育手机客户端 说明
一般描述
Protein tyrosine phosphatase. Catalytic domain, (amino acids 839-1100), (Gene bank accession number BC051651) with N-terminal GST tag, MW = 56.2 kDa, expressed in anE. coliexpression system.
应用
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
单位定义
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed inE. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 56.2 kDa |
| 包装 | pkg of 20 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | BC051651 |
| UniProt登记号 | P28827 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... PTPRM(5797) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
