半岛bd体育手机客户端 说明
一般描述
Protein tyrosine phosphatase, catalytic domain (amino acids. 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in anE. coliexpression system.
应用
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
单位定义
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.
外形
Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
半岛bd体育手机客户端 性质
| 生物来源 | rat |
| 重组 | expressed inE. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 63.3 kDa |
| 包装 | pkg of 20 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_012637.2 |
| UniProt登记号 | P20417 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... PTPN1(5770) |
Sigma-Aldrich
m.cnreagent.com
