PP2A ALPHA/PPP2R1A Complex, Active

半岛bd体育手机客户端 说明

应用

Useful for Phosphatase assay and Western blot.

生化/生理作用

Recombinant full length human PP2Aalpha and human PPP2R1A were expressed byBaculovirusin Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A⁽¹⁾. Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity⁽²⁾.

重悬

Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	3400-4600 nmol/min·mg (BIOMOL Green)
分子量	~38 kDa ~65 kDa
浓度	0.1 μg/μL
登记号	NM_002715 NM_014225
运输	dry ice
储存温度	?70℃
Gene Information	human ... PP2A(5524), PPP2R1A(5518)

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H360D
预防措施声明	P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
危险分类	Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable