Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

半岛bd体育手机客户端 说明

一般描述

PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

细胞系来源

Artery

应用

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

组分

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

制备说明

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

传代培养常规

Please refer to the PPAEC Culture Protocol.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

生物来源	Porcine pulmonary artery (Normal healthy)
质量水平	100
形式	solid
包装	pkg of 500,000 cells
生长模式	Adherent
核型	2n = 38
形态学	Endothelial
technique(s)	cell culture | mammalian: suitable
相关疾病	diabetes; cardiovascular diseases
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable