pNPP Phosphatase Assay Kit

半岛bd体育手机客户端 说明

一般描述

Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A₄₀₅nm on a spectrophotometer.

特点和优势

• No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.

原理

The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 ℃.

半岛bd体育手机客户端 性质

质量水平	100
detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H302 + H332 - H314 - H341 - H373 - H411
预防措施声明	P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3