PLK1 Polo Box Domain active human

半岛bd体育手机客户端 说明

一般描述

Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in anE. coliexpression system.
Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2.

应用

PLK1 Polo Box Domain active human has been used in thein vitrobinding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQSpTPLNG to test the effect of various inhibitors.

生化/生理作用

Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	aqueous solution
分子量	53 kDa
包装	pkg of 100 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	2.9 mg/mL
NCBI登记号	NM_005030
UniProt登记号	P53350
运输	dry ice
储存温度	?70℃
Gene Information	human ... PLK1(5347)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable