PIP5K3 (PIKFYVE), Active

半岛bd体育手机客户端 说明

应用

Useful for kinase assay.

生化/生理作用

Recombinant human PIP5K3 (1400-end) was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity⁽¹⁾. PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies⁽²⁾.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

推荐半岛bd体育手机客户端

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	90-115 nmol/min·mg (ADP-Glo)
分子量	~110 kDa
浓度	0.05-0.1 μg/μL
登记号	NM_015040
运输	dry ice
储存温度	?70℃
Gene Information	human ... PIP5K3(200576)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable