PI3K (P85 ALPHA) Protein

半岛bd体育手机客户端 说明

应用

Useful for Western blot and kinase assays.

生化/生理作用

Recombinant full-length human PI3K (p85alpha) was expressed byBaculovirusin Sf9 insect cells using an N-terminal His tag. The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PI3K and platelet-derived growth factor receptor⁽¹⁾. Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS)⁽²⁾.

重悬

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
分子量	~86 kDa
浓度	0.2 μg/μL
登记号	NM_181523
运输	dry ice
储存温度	?70℃
Gene Information	human ... PI3K (5295)

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H360D
预防措施声明	P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
危险分类	Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable