PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

半岛bd体育手机客户端 说明

一般描述

This PhosphoDetect Anti-p53 (pSer??) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer??) (Ab-3).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser¹⁵.


Recognizes the ~53 kDa p53 protein phosphorylated at Ser¹⁵in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.

免疫原

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser?? phosphorylation site of human p53, conjugated to KLH

包装

50 μL in Plastic ampoule

应用

Immunoblotting (1:1000)
Immunoprecipitation (1:200)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium HEPES, 100 ?g/ml BSA, 50% glycerol, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

分析说明

Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents

其他说明

Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999.Genes Dev.13,152.
Levine, A.J. 1997.Cell88,323.
Milczarek, G.J., et al. 1997.Life Sci.60,1.
Shieh, S.Y., et al. 1997.Cell91,325.
Meek, D.W. 1994.Semin Cancer Biol.5,203.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	liquid
不包含	preservative
species reactivity	human, mouse, rat
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2