PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

半岛bd体育手机客户端 说明

一般描述

This PhosphoDetect Anti-NBS1 (pSer???) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer???).
Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³in δ-irradiated lymphoblastoid cells.
Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.

免疫原

a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser??? phosphorylation site of human nibrin, conjugated to diphtheria toxoid
Human

包装

50 μL in Plastic ampoule

应用

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 ?l/500 ?g cell extract)

警告

Toxicity: Standard Handling (A)

外形

Serum diluted in PBS.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
γ-irradiated lymphoblastoid cells

其他说明

Gatei, M., et al. 2000.Nat. Genet.25,115.
Lim, D.S., et al. 2000.Nature404,613.
Wu, G., et al. 2000.J. Biol. Chem.275,30618.
Zhong, Q., et al. 1999.Science285,747.
Carney, J.P., et al. 1998.Cell93,477.
Trujillo, K.M., et al. 1998.J. Biol. Chem.273,21447.
Varon, R., et al. 1998.Cell93,467.
This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	diluted serum
antibody product type	primary antibodies
克隆	polyclonal
形式	liquid
不包含	preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable