PFTK1 (CDK14)/CYCLINY, Active

半岛bd体育手机客户端 说明

应用

Useful for Kinase assay and Western blot.

生化/生理作用

Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed byBaculovirusin Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system⁽¹⁾. PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding⁽²⁾. PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	2.0-3.5 nmol/min·mg (radiometric)
分子量	~64 kDa (Cyclin Y) ~72 kDa (PFTK1 (CDK14))
浓度	0.1 μg/μL
登记号	NM_012395 NM_145012
运输	dry ice
储存温度	?70℃
Gene Information	human ... CCNY(219771), CDK14(5218)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable