PFKFB3 Protein

半岛bd体育手机客户端 说明

应用

Useful for Western blot.

生化/生理作用

Recombinant full-length human PFKFB3 was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. PFKFB3 or 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 is a potent activator of a key regulatory enzyme in the glycolysis pathway called phosphofructokinase. Phosphofructokinase regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of key regulatory enzyme of the glycolysis pathway. PFKFB3 is bifunctional, although the phosphatase activity is low due to a mutation in an active site which can be upregulated after phosphorylation by protein kinases⁽¹⁾. PFKFB3 may be responsible for sustaining the high glycolytic flux of rapidly proliferating leukemia cells and may represent a molecular target for chemotherapeutic intervention⁽²⁾.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	75-95% (SDS-PAGE)
形式	frozen liquid
分子量	~83 kDa
浓度	0.1 μg/μL
登记号	NM_004566
运输	dry ice
储存温度	?70℃
Gene Information	human ... PFKFB3(5209)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable