半岛bd体育手机客户端 说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed byBaculovirusin Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons(1). The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins(2).
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
半岛bd体育手机客户端 性质
重组 | expressed in baculovirus infectedSf9 cells |
测定 | 70-95% (SDS-PAGE) |
形式 | frozen liquid |
specific activity | 15-25 nmol/min·mg (radiometric) |
分子量 | ~64 kDa (Cyclin Y) ~88 kDa (PCTK2) |
浓度 | 0.1 μg/μL |
登记号 | NM_002595 NM_145012 |
运输 | dry ice |
储存温度 | ?70℃ |
Gene Information | human ... CCNY(219771), CDK17(5128) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich