半岛bd体育手机客户端 说明
一般描述
Human PARP9 (GenBank Accession No. NM_031458), full length with N-terminal GST tag, MW = 119 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 119 kDa |
| 包装 | pkg of 10 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_031458 |
| UniProt登记号 | Q8IXQ6 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... PARP9(83666) |
Sigma-Aldrich
m.cnreagent.com
