MULTI-seq Lipid-Modified Oligos

半岛bd体育手机客户端 说明

应用

MULTI-seq, multiplexing using lipid-tagged indices, is a novel tool that facilitates multiplex single-cell and single-nucleus RNA sequencing1. This technology provides sample barcoding that is compatible with any cell type or nucleus containing an accessible plasma membrane and allows for streamlined, pooled single-cell sample processing.

特点和优势

• Efficient.MULTI-seq increases the throughput and decreases the reagent cost of droplet-based single-cell RNA sequencing.
• Flexible.Compatible with any droplet generation platform, the indexing system enables users to run and analyze up to 96 barcoded samples simultaneously in an 8-lane droplet device.
• Clearer Results.The indices provide data quality advantages over non-indexed analysis methods in the form of doublet identification and retention of data from cells with low RNA content.

组分

The MULTI-seq Lipid Modified Oligos kit is comprised of a lignoceric amide-modified anchor DNA oligo solution and a palmitic amide-modified co-anchor DNA oligo solution. Together, these lipid-modified oligos embed into cell or nuclei membranes and provide a landing pad for DNA barcodes with a complementary 5′ sequence. The MULTI-seq Lipid Modified Oligos kit is intended for upstream sample preparation only, before pooling and single cell analysis.

NOTE: Unique barcode oligos are not included and must be purchased separately.

Barcode 3′ oligo design with poly-A tail for mRNA enrichment1:
5′-CCTTGGCACCCGAGAATTCCA-8-base index-A30-3′

Barcode oligo design for 5′ cDNA library synthesis:
5′-CCTTGGCACCCGAGAATTCCA-8-base index-CCCATATAAGAAA-3′

Custom barcode oligos may be ordered from SigmaAldrich.com/nextgenoligos. For minimal cross-contamination of barcode primers, order Next-Gen Sequencing Oligos with NGSO-Silver or NGSO-Gold quality.

Reagents Provided

• LMO001ALignoceric Anchor with DNA Oligo
• LMO001BPalmitic Co-anchor with DNA Oligo

Each lipid-modified oligo is supplied at a concentration of 50 μM in water.

半岛bd体育手机客户端 性质

质量水平	200
形式	liquid
用途	sufficient for 100 sample(s) (100RXN kit is adequate for labelling 100 samples when using 1 μL of each LMO per sample) sufficient for 30 sample(s) (30RXN kit is adequate for labelling 30 samples when using 1 μL of each LMO per sample)
浓度	50 μM (each)
technique(s)	multiplexing: suitable (Single Cell and Single Nucleus)
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1