Monoclonal Anti-SPAG11 antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

SPAG11A (sperm associated antigen 11A) belongs to the β defensin family. The protein is mainly expressed in the epididymis. The gene is mapped to human chromosome 8.
Monoclonal Anti-SPAG11 (mouse IgG1 isotype) is derived from the hybridoma 10B4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. SPAG11 is expressed specifically in epithelial cells in the middle part of the caput region. It exists in four isoforms such as A, D, E and G.

特异性

Monoclonal Anti-SPAG11 recognizes mouse and rat SPAG11.

免疫原

a recombinant rat SPAG11

应用

Monoclonal Anti-SPAG11 antibody produced in mouse may be used in immunoblotting and immunohistochemistry.

生化/生理作用

SPAG11A (sperm associated antigen 11A) is a secretory protein with possible involvement in sperm maturation. It also controls inflammatory responses in the epididymis.
SPAG11 is involved in promoting sperm motility due to its capability to bind the sperm head in different regions of the epididymis. It exhibits anti-microbial activity by suppressing colony growth ofEscherichia coliand other Gram-negative bacteria.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For extended storage, freeze at -20 ℃ in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezer is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	10B4, monoclonal
形式	buffered aqueous solution
分子量	monomer ~7 kDa ~34 kDa (multimer)
species reactivity	mouse, rat
浓度	~1 mg/mL
technique(s)	immunoblotting: 4-8 μg/mL using whole extracts of HEK-293 cells overexpressing rat SPAG11 immunohistochemistry: 5-10 μg/mL using paraffin-embedded rat caput epididimys sections
同位素/亚型	IgG1
运输	dry ice
储存温度	?20℃
Gene Information	mouse ... Spag11a(78128) rat ... Spag11a(246305)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable