半岛bd体育手机客户端 说明
一般描述
The mobilization region of the 7.4 kb beta-lactamase plasmid pSJ7.4 from Neisseria gonorrhoeae was characterized. The 3.2 kb HindIII-BamHI fragment of pSJ7.4 was mobilized between Escherichia coli strains by conjugative plasmid RK2. Selected restriction enzyme-generated deletions of this fragment were subcloned in pACYC177 to obtain constructs that were suitable for analysis of the mobilization region. Mating experiments showed that a highly conserved 1.9 kb DNA region within coordinates 4096-5997 is required for mobilization of pSJ7.4. This region contains two genes encoding the mobilization protein MobA, another protein, MobC, putatively involved in mobilization from some hosts, and an intergenic oriT. The 168 bp intergenic sequence also contains the promoters for mobA and mobC in an arrangement that suggests divergent transcription and autoregulation from oriT. The 56 kDa MobA was expressed in E. coli as a (6x)His-Tag fusion protein. Purified MobA specifically induced plasmid relaxation by nicking at the oriT. MobA is exceptional because the N-terminal region alone can mobilize pSJ7.4, albeit at a lower frequency than the full-length protein, even in the absence of MobC. The carboxyl terminal region of MobA did not share homology with other mobilization proteins, but may be involved in promoting efficient transfer of pSJ7.4.
免疫原
MOBC (AAO45531, )
This antibody was raised using purified His-tagged recombinant full length Neisseria gonorrhoeae plasmid pSJ7.4 mobilization protein C (mobC)and mobilization protein A (mobA).
外形
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
基本信息
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 19CT.2.8.14, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | Neisseria gonorrhoeae |
| technique(s) | indirect ELISA: 1:1000 western blot: 1:100-1:500 |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | AAO45531 |
| UniProt登记号 | Q84AT5 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
