Monoclonal Anti-MASTL antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-MASTL (mouse IgG2b isotype) is derived from the hybridoma 2C8/D11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. Microtubule-Associated Serine/Threonine kinase-Like (MASTL), also known as GREATWALL (GWL) inDrosophilaandXenopusis localized to the nucleus but, during M-phase it is activated and re-localized to the centrosomes.

免疫原

immunized with a fusion protein of 250aa polypeptide from the region that divides the kinase domain of mouse MASTL

应用

Monoclonal Anti-MASTL antibody produced in mouse may be used in various immunochemical techniques including immunoblotting, flow cytometry and immunofluorescence.

生化/生理作用

Microtubule-associated serine/Threonine kinase-Like (MASTL) acts as a cell cycle marker of M phase. It has an ability to regulate mitosis entry, anaphase and cytokinesis. In Drosophila MASTL (Greatwall) was initially identified as a kinase essential for chromosome condensation. MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events. Furthermore, MASTL is also an inhibitor of the tumor suppressor protein Phosphatase 2A (PP2A), and thus maintains the equilibrium between cyclin B-Cdk1 and PP2A. A missense mutation in the MASTL gene leads to the development of autosomal dominant inherited thrombocytopenia.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	2C8/D11, monoclonal
形式	buffered aqueous solution
分子量	~100 kDa
species reactivity	monkey, human
浓度	~1 mg/mL
technique(s)	flow cytometry: 10-20 μg/test using using human HeLa cells. immunoblotting: 1-2 μg/mL using whole extracts of human HeLa cells. immunofluorescence: 10-20 μg/mL using using human HeLa cells.
同位素/亚型	IgG2b
UniProt登记号	Q96GX5
运输	dry ice
储存温度	?20℃
Gene Information	mouse ... Mastl(67121)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable