半岛bd体育手机客户端 说明
免疫原
Immunogen Region: Other – provide in Additional Product Details
Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
Immunogen Species: Human
Immunogen type: Recombinant protein
应用
Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate
Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa
外形
50% glycerol, 0.09% Sodium Azide
PBS pH 7.4
制备说明
Derived from cell/tissue culture
储存及稳定性
Protect from light; avoid repeated freeze-thaw cycles. Store at -20 ℃ long-term, 2-8 ℃ short-term.
其他说明
Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase
Target/Gene Description: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.45 |
半岛bd体育手机客户端 性质
生物来源 | mouse |
偶联物 | Atto 488 conjugate |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | 4F9 monoclonal |
描述 | clone 4F9, purified immunoglobulin, buffered aqueous solution |
形式 | buffered aqueous solution |
species reactivity | human (Tested) |
包装 | pkg of 100 μg |
储存条件 | avoid repeated freeze/thaw cycles protect from light |
浓度 | 1.0 mg/mL |
靶分子量 | 57 kDa |
technique(s) | immunofluorescence: 1:100 western blot: 1:1000 |
同位素/亚型 | IgG2b |
UniProt登记号 | P30101 |
运输 | wet ice |
储存温度 | ?20℃ |
Gene Information | human ... PDIA3(2923) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich