Monoclonal Anti-ERp57-Atto 390 antibody produced in mouse

半岛bd体育手机客户端 说明

免疫原

Immunogen type: Recombinant protein
Immunogen Species: Human
Immunogen Region: See Notes section/Other
Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

应用

Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa

目标描述

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .

外形

50% glycerol, 0.09% Sodium Azide
PBS pH 7.4

制备说明

Derived from cell/tissue culture

储存及稳定性

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 ℃ long-term, 2-8 ℃ short-term.

其他说明

Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase

Other:
Immunogen region: Amino acids 25-505
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.45

半岛bd体育手机客户端 性质

生物来源	mouse
偶联物	Atto 390 conjugate
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	4F9 monoclonal
形式	buffered aqueous solution
species reactivity	human (Tested)
包装	pkg of 100 μg
浓度	1.0 mg/mL
靶分子量	57 kDa
technique(s)	immunofluorescence: 1:100 western blot: 1:1000
同位素/亚型	IgG2b
Protein ID登记号	NP_005304
UniProt登记号	P30101
运输	wet ice
储存温度	?20℃
Gene Information	human ... PDIA3(2923)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2