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位置: 首页> 品牌> Sigma-Aldrich> Monoclonal Anti-CD8-FITC antibody produced in mouse

Monoclonal Anti-CD8-FITC antibody produced in mouse

品牌
Sigma-Aldrich
货号
F0772
规格纯度
clone UCHT-4, purified immunoglobulin, buffered aqueous solution
价格
4731.57 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Monoclonal Anti-CD8-FITC antibody produced in mouse
Monoclonal Anti-CD8
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with human thymocytes followed by peripheral blood T cells. The CD8 antigen is strongly expressed on approximately one-third of mature T cells (cytotoxic/suppressor T cells). Monoclonal Anti-CD8 does not stain B lymphocytes, monocytes, or granulocytes.

特异性

Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567

免疫原

human thymocytes followed by peripheral blood T cells.

应用

FITC Monoclonal Anti-Human CD8 may be used for:

  • Immunofluorescent staining,
  • Enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids,
  • Identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues,
  • Analysis of cell mediated cytotoxicity,
  • Studies of immunoregulation in health and disease,
  • Investigation of NK cells, Complement mediated cytolysis of CD8 expressing cells

生化/生理作用

CD8a (Cluster of Differentiation 8a) is a human gene. CD8 bind to class I MHC and the co-receptors during the process of cytotoxic T-cell activation. T cells that express T cell receptor (TCR) complexes, which lack CD3 δ, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 δ couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8+T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).

目标描述

CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lckprotein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

制备说明

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.44

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 FITC conjugate
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 UCHT-4, monoclonal
形式 buffered aqueous solution
species reactivity human
technique(s) flow cytometry: 10 μL using 1 × 106cells
同位素/亚型 IgG2a
UniProt登记号 P10966
运输 wet ice
储存温度 2-8℃
Gene Information human ... CD8A(925), CD8B(926)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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