MLK4, Active

半岛bd体育手机客户端 说明

应用

Useful for kinase assay and Western blot.

生化/生理作用

Recombinant human MLK4 (114-420) was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas⁽¹⁾. MLK4 is a negative regulator of TLR4⁽²⁾.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	15-25 nmol/min·mg (radiometric)
分子量	~61 kDa
浓度	0.1 μg/μL
登记号	NM_032435
运输	dry ice
储存温度	?70℃
Gene Information	human ... MLK4(84451)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable