半岛bd体育手机客户端 说明

一般描述

MEKK1 or MEK kinase, is a serine/threonine kinase that is downstream of mitogenic and metabolic stimuli, including insulin and many growth factors. MEKK1 functions not only as an upstream activator of ERK and JNK through its kinase domain, but also as an E3 ligase through its PHD domain, providing a negative regulatory mechanism for decreasing ERK1/ERK2 activity by ubiquitination and degradation. MEKK1 -/- embryonic stem cells from mice show loss or altered responses of JNK to microtubule disruption and cold stress. Furthermore, activation of JNK is lost and that of ERK is diminished in response to hyperosmolarity and serum factors in MEKK1 -/- cells.

外形

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

制备说明

after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
半岛bd体育手机客户端 线	PRECISIO?Kinase
测定	≥70% (SDS-PAGE)
形式	buffered aqueous glycerol solution
specific activity	697-943 nmol/min·mg
分子量	~155 kDa
NCBI登记号	NM_005921
运输	dry ice
储存温度	?70℃
Gene Information	human ... MAP3K1(4214)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1