LIMK2, Active

半岛bd体育手机客户端 说明

应用

Useful for kinase assay and Western blot.

生化/生理作用

Recombinant full-length human LIMK2 was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. LIMK2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain⁽¹⁾. LIM domains are highly conserved cysteine-rich structures that can mediate protein-protein interaction. LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and then LIMK2 in turn phosphorylates cofilin, inhibiting its actin-depolymerizing activity which contributes to Rho-induced reorganization of the actin cytoskeleton⁽²⁾. LIMK2 is highly expressed in liver, colon, stomach, spleen, brain, kidney, and placenta.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	2.5-3.5 nmol/min·mg (radiometric)
分子量	~96 kDa
浓度	0.05 μg/μL
登记号	CR456513
运输	dry ice
储存温度	?70℃
Gene Information	human ... LIMK2(3985)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable