半岛bd体育手机客户端 名称:
L
-Lactate Assay Kit
半岛bd体育手机客户端 介绍:
半岛bd体育手机客户端 说明
一般描述
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
特点和优势
- Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
- Convenient room temperature assay. The procedure involves adding a single working reagent, and reading the fluorescence after 60 minutes.
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for the quantitative determination of
L
-lactate (
L
-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
原理
The
L
-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
半岛bd体育手机客户端 性质
质量水平 |
100 |
detection method |
fluorometric |
相关疾病 |
diabetes; pulmonary disorders; cardiovascular diseases; cancer; endocrinological disorders |
储存温度 |
?20℃ |
安全信息
储存分类代码 |
10 - Combustible liquids |
WGK |
WGK 2 |