半岛bd体育手机客户端 说明
一般描述
Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥40% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 57 kDa |
| 包装 | pkg of 100 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_015015 |
| UniProt登记号 | O94953 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... KDM4B(23030) |
Sigma-Aldrich
m.cnreagent.com
