Human Ferritin ELISA Kit

半岛bd体育手机客户端 说明

一般描述

The Human Ferritin ELISA (Enzyme-Linked Immunosorbent Assay) kit is anin vitroenzyme-linked immunosorbent assay for the quantitative measurement of human Ferritin in serum, plasma, cell culture supernatants and urine.
Ferritin is a heteropolymer globular protein of 450 kDa and is localized to the nucleus, mitochondria and cytoplasm. Ferritin is an intracellular protein containing 24 subunits (heavy and light).

免疫原

Recombinant Human Ferritin

应用

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human ferritin ELISA kit. has been used to measure the ferritin secreted in supernatants from THP-1 (tamm-horsfall protein 1) cells that were sequentially treated with lipopolysaccharides (LPS) and LLOMe (a lysosome disruption agent).
Human Ferritin ELISA Kit has been used to estimate the amount of ferritin in cellular suspension.

生化/生理作用

Ferritin/ ferritin heavy chain 1 (FTH1) is a cytoplasmic protein. It acts as a cargo for transport to autophagosomes for degradation. FTH1 maintains the cellular redox status by storing the intracellular iron in a non-toxic and readily available form. It acts as a key antioxidant molecule in various physiologic and pathologic conditions, such as apoptosis, inflammation, vascular protection and neurodegeneration. FTH1 alone or along with ferritin light chain is implicated to detect cancer cells, stem cells, fibroblasts and dendritic cells.
Ferritin mediates iron metabolism and plays a key role in redox homeostasis. Ferritin possesses ferroxidase activity and maintains iron in its non-toxic form. Ferritin acts as an iron-storage protein and stores excessive intracellular iron that is not utilized for the production of heme. Also, iron is released from ferritin upon degradation.

其他说明

A sample Certificate of Analysis is available for this product.
Please type the wordsamplein the text box provided for lot number.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

species reactivity	human
包装	kit of 96 wells (12 strips x 8 wells)
technique(s)	ELISA: suitable capture ELISA: suitable
input	sample type plasma sample type cell culture supernatant(s) sample type urine sample type serum
assay range	inter-assay cv: <12% intra-assay cv: <10% sensitivity: 600 pg/mL standard curve range: 0.81-200 ng/mL
detection method	colorimetric
运输	wet ice
储存温度	?20℃
Gene Information	human ... FTH1(2495)

组份列表

组份可单独销售

货号	组份
RABELADA	ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
RABELADB	ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
RABSTOP3	ELISA Stop Solution (Item I)
RABTMB3	ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
RABWASH4	20X Wash Buffer (Item B)

安全信息

象形图
警示用语：	Warning
危险声明	H290
预防措施声明	P234 - P390
危险分类	Met. Corr. 1
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3