半岛bd体育手机客户端 说明
一般描述
Hsp90b (heat shock protein 90-β) is a constitutive cytosolic isoform of Hsp90. The gene is mapped to human chromosome 6p21.1. Hsp90b is an ATP-dependent molecular chaperone and is upregulated in many tumor cells.
Human Heat Shock Protein 90β (GenBank Accession No. AY359878), full length with C-terminal His tag, MW = 83kDa, expressed in anEscherichia coliexpression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
Hsp90 (heat shock protein 90) is needed for folding, maturation, transport, and degradation of various proteins, particularly signaling molecules, steroid receptors, and transcription factors. Hsp90b (Hsp90 β) is secreted by various cells and works as a chaperone for selected surface-associated or secreted proteins. It acts as a chaperone for matrix metalloproteinases and extracellular matrix proteins, and helps in cell motility. Suppression of the Hsp90b activity is suggested as a therapeutic approach for various cancers and inflammatory diseases.
外形
Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed inE. coli |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 83 kDa |
| 包装 | pkg of 200 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | AY359878 |
| UniProt登记号 | P08238 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... HSP90AB1(3326) |
Sigma-Aldrich
m.cnreagent.com
