HRAS1 (G12V) Protein

半岛bd体育手机客户端 说明

生化/生理作用

Recombinant human RAS1 (2-186, G12V) was expressed inE.colicells using an N-terminal His tag. The HRAS1(G12V) mutant is constitutively active. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking⁽¹⁾. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types⁽²⁾. Other members of the RAS superfamily may also contribute to cancer.

序列

2-186

重悬

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
分子量	~84 kDa
浓度	0.1 μg/μL
登记号	NM_003159
运输	dry ice
储存温度	?70℃
Gene Information	human ... HRAS1(6792)

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H360D
预防措施声明	P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
危险分类	Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable