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Histone H3 (2-58) human

品牌
Sigma-Aldrich
货号
SRP0158
规格纯度
recombinant, expressed inE. coli, ≥70% (SDS-PAGE)
价格
6302.26 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Histone H3 (2-58) human
Histone H3.1
HIST1H3E
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)2. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).
Human Histone 3 (GenBank Accession No. NM_003532), (amino acid 2-58) with N-terminal GST tag, MW = 32kDa, expressed in anEscherichia coliexpression system.

应用

Suitable substrate for histone methyltransferases

生化/生理作用

Histone proteins are basic building blocks of chromatin. H3.1 (also referred to as HIST1H3E) is mainly expressed in the S phase and is termed as replication-dependent histone. Selective methylation of H3.1 controls replication in heterochromatin area.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES NA.32

半岛bd体育手机客户端 性质

生物来源 human
重组 expressed inE. coli
测定 ≥70% (SDS-PAGE)
形式 aqueous solution
分子量 32 kDa
包装 pkg of 500 μg
储存条件 avoid repeated freeze/thaw cycles
浓度 >0.02 mg/mL
NCBI登记号 NM_003532
UniProt登记号 P68431
运输 dry ice
储存温度 ?70℃
Gene Information human ... HIST1H3E(8353)

Sigma-Aldrich

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