Glutathione S-transferase Assay Kit

半岛bd体育手机客户端 说明

一般描述

Glutathione transferase (GST) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.

特点和优势

• Fast and sensitive. Linear detection range (20 μL sample): 2 to 80 U/L for 10 min reaction at 25 ℃.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc.

原理

The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at A₃₄₀ nm. The rate of increase in absorbance at A₃₄₀ nm is directly proportional to the GST activity in the sample.

半岛bd体育手机客户端 性质

安全信息

象形图
警示用语：	Warning
危险声明	H317 - H319 - H412
预防措施声明	P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	188.6 °F
闪点(C)	87 ℃