Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

半岛bd体育手机客户端 说明

一般描述

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

特点和优势

• Compatible with high-throughput handling systems.

适用性

The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

原理

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅nm. The increase in absorbance at A₅₆₅nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

半岛bd体育手机客户端 性质

质量水平	100
detection method	colorimetric
相关疾病	hematological disorder, anemia; cancer
储存温度	?20℃

安全信息

象形图
警示用语：	Warning
危险声明	H341
预防措施声明	P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类	Muta. 2
储存分类代码	10 - Combustible liquids
WGK	WGK 3