Glucose Dehydrogenase Assay Kit

半岛bd体育手机客户端 说明

一般描述

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

特点和优势

• Linear detection range (20 μL sample): 0.5 to 200 U/L for 15 minute reaction.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the quantitative determination of D -glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

原理

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

半岛bd体育手机客户端 性质

质量水平	100
detection method	colorimetric
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H225 - H290 - H315 - H319 - H336
预防措施声明	P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
靶器官	Central nervous system
储存分类代码	3 - Flammable liquids
WGK	WGK 2