半岛bd体育手机客户端 说明
一般描述
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
单位定义
One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30℃. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30℃ for 30 min.
外形
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human pancreas |
| 重组 | expressed inE. coli |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | liquid |
| 分子量 | 53.1 kDa |
| 包装 | pkg of 10 μg |
| 浓度 | 1 mg/mL |
| 杂质 | Endotoxin, tested |
| NCBI登记号 | P35557 |
| UniProt登记号 | P35557 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... GCK(2645) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
