GAL4 [(1-147)-AH] fromSaccharomyces cerevisiae

半岛bd体育手机客户端 说明

生化/生理作用

Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in anin vitrotranscription system by targeting TFIIB in the pre-initiation complex.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

基本信息

NACRES

NA.26

半岛bd体育手机客户端 性质

生物来源	Saccharomyces cerevisiae
重组	expressed inE. coli
测定	≥80% (SDS-PAGE)
形式	frozen liquid
分子量	~18.6 kDa
包装	pkg of 10 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	300 μg/mL
technique(s)	electrophoretic mobility shift assay: suitable
颜色	colorless to clear
NCBI登记号	NM_001184062
UniProt登记号	P04386
运输	dry ice
储存温度	?70℃
Gene Information	Saccharomyces cerevisiae... GAL4(855828)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable