Furin human

半岛bd体育手机客户端 说明

一般描述

Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg′). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.

外形

Lyophilized from 0.22 μm filtered solution in MES, Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

重悬

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in HEK 293 cells
标签	6-His tagged (C-terminus)
测定	≥95% (SDS-PAGE)
形式	lyophilized powder
分子量	calculated mol wt 66.5 kDa observed mol wt 55-66 kDa (DTT-reduced. Asp 108 is the predicted N-terminus .)
包装	pkg of 10 μg
杂质	<1 EU/μg endotoxin (LAL test)
UniProt登记号	P09958
运输	wet ice
储存温度	?20℃
Gene Information	human ... PACE(5045)

安全信息

危险声明	H412
预防措施声明	P273 - P501
危险分类	Aquatic Chronic 3
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable