半岛bd体育手机客户端 说明
一般描述
Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW = 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 51 kDa (EED) 86 kDa (EZH2) 87 kDa (SUZ12) |
| 包装 | pkg of 50 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_003797 (EED) NM_004456 (EZH2) NM_015355 (SUZ12) |
| UniProt登记号 | Q15910 |
| 运输 | dry ice |
| 储存温度 | ?70℃ |
| Gene Information | human ... EZH2(2146) |
Sigma-Aldrich
m.cnreagent.com
