ESGRO^?Recombinant Rat LIF Protein

半岛bd体育手机客户端 说明

一般描述

Product Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
Applications for Rat ESGRO include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.

应用

Applications for Rat ESGRO include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et.al. 1998).

Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et.al. 1998).

At this recommended concentration 10⁶units of ESGRO is sufficient for 1L of tissue culture media.

Visit www.esgro-lif.com for additional information

Rat LIF is not available for resale.

For research use only; not for use as a diagnostic.

包装

1 mL

质量

The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.

注意

Note: LIF should not be allowed to come in contact with ruminant or swine.

外形

Purified, sterile liquid. Rat ESGRO is supplied in liquid form as 10⁶Units ESGRO in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability.



Rat ESGRO is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.
Format: Purified

储存及稳定性

Rat ESGRO is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells.



For long term storage it is recommended that Rat ESGRO concentrate be stored at -20°C.



It is recommended that prior to use, Rat ESGRO should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at -20°C. Freeze thawing will reduce potency and is not recommended.

分析说明

Specific Activity: Rat ESGRO^?is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells.A standard of 50 Units is defined as the concentration of Rat ESGRO in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies.10⁶units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media.Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity >10⁸units/mg

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160801
NACRES	NA.75

半岛bd体育手机客户端 性质

质量水平	100
测定	>85% (active component rat LIF (rtLIF), SDS-PAGE)
形式	liquid
manufacturer/tradename	Chemicon?
浓度	1000000 units/mL
technique(s)	cell culture | stem cell: suitable
input	sample type: mouse embryonic stem cell(s) sample type induced pluripotent stem cell(s)
UniProt登记号	P17777
运输	dry ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2