Escherichia coli FISH probe-Cy3

半岛bd体育手机客户端 说明

一般描述

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells.1 Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.2

FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood3 and tissue4), microbial ecology (solid biofilms5 and aquatic systems 6) and plants7. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest.

Escherichia coliis a gram negative, facultative aerobic, rod-shaped coliform bacterium.E. colicolonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction.8,9 Nevertheless,E. colican survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats ofE. coliinclude soil, water, sediment, and food. Some strains ofE. colihave evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.10

Escherichia coliprobe specifically recognizesEscherichia colicells.

Yet there are some reports that describe recognition of other bacteria with this probe, such as,Shigella boydii,Citrobacter davisae,Citrobacter lapagei,Citrobacter neteri11 andKlebsiella pneumoniae12.

FISH technique was successfully used to identifyE.coliwith the probe in various samples such as pure culture (as described in the figure legends and11,13), large and small intestines samples14-16, fecal samples17-21, colonic biopsies18, urine samples, bladder and kidney sections embedded in paraffin22 and inE.colibiofilm23.

应用

Probe for fluorescencein situhybridization (FISH),recognizesEscherichia colicells

特点和优势

• Visualize, identify and isolateEscherichia colicells.
• Observe nativeE. colicell populations in diverse microbiome environments.
• Specific, sensitive and robust identification ofE. coliin bacterial mixed population.
• Specific, sensitive and robust identification even whenE. coliis in low abundance in the sample.
• FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information onE.colimorphology and allows to study biofilm architecture.
• IdentifyE.coliin clinical samples such as, urine samples, bladder and kidney sections (formalin-fixed paraffin-embedded (FFPE) samples), fecal samples and colon tissue.
• The ability to detectE.coliin its natural habitat is an essential tool for studying host-microbiome interaction.

半岛bd体育手机客户端 性质

质量水平	200
保质期	2 yr
荧光	λex550 nm; λem570 nm
运输	dry ice
储存温度	?20℃