EPA Peroxidase Protection Buffer

半岛bd体育手机客户端 说明

一般描述

Peroxidase Protective Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.

应用

USE:

Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in Peroxidase Protective Buffer. Diluted conjugates are stable for at least 3 months when stored at 2 - 8oC. The buffer is suitable for direct use in ELISA. It is recommended that the HRP conjugated antibody is diluted with Peroxidase Protective Buffer and allowed to stand for 24 hours at 2-8°C to attain constant stability before use.

Peroxidase Protective Buffer is compatible with blocking agents such as skim milk powder, Tween 20 and normal serum. However HRP conjugates should not be stored in the presence of these blocking agents. They should be added just prior to use in the assay.

外形

Peroxidase Protective Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2.

储存及稳定性

Store at +2 to +8°C, for up to 6 months.

PRECAUTIONS

HRP conjugates should be diluted and stored under clean conditions to reduce the level of microbial challenge.

Sodium azide must NOT be added to Peroxidase Protective Buffer.

Avoid contamination of HRP conjugate solutions with heavy metal ions, aromatic chlorocarbons or hypochlorous acids. Avoid containers made of soda glass or polystyrene. Storage of diluted conjugates in borosilicate glass is recommended.

Efficient washing steps should follow HRP conjugate incubations. In ELISA, at least four thorough wash cycles are recommended.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	42029053
NACRES	NB.22

半岛bd体育手机客户端 性质

包装	pkg of 500 mL
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable
运输	wet ice

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H319 - H412
预防措施声明	P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
危险分类	Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable