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eIF4E Active human

品牌
Sigma-Aldrich
货号
SRP0255
规格纯度
N-terminal GST-tag, recombinant, expressed inE. coli, ≥70% (SDS-PAGE)
价格
8831.52 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
eIF4E Active human
mRNA cap-binding protein
Eukaryotic translation initiation factor 4E
CBP
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein. It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.

应用

Suitable substrate for MNK1 kinase.

生化/生理作用

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.

外形

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES NA.32

半岛bd体育手机客户端 性质

生物来源 human
重组 expressed inE. coli
测定 ≥70% (SDS-PAGE)
形式 aqueous solution
分子量 51 kDa
包装 pkg of 100 μg
浓度 >0.02 mg/mL
NCBI登记号 NM_001968
UniProt登记号 P06730
运输 dry ice
储存温度 ?70℃
Gene Information human ... EIF4E(1977)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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