EGFR (D746-750 T790M C797S), Active

半岛bd体育手机客户端 说明

应用

Useful for kinase assay.

生化/生理作用

Recombinant human EGFR (d746-750 T790M C797S) (668-end) was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival⁽¹⁾. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms⁽²⁾. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	40-56 nmol/min·mg (radiometric)
分子量	~89-100 kDa
浓度	0.1 μg/μL
登记号	NM_005228
运输	dry ice
储存温度	?70℃
Gene Information	human ... EGFR(1956)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable