Duolink^?PLA^?Probe Mouse Anti-PhosTyr MINUS from mouse

半岛bd体育手机客户端 说明

一般描述

Anti-Phosphotyrosine antibody, clone 4G10^?with the Duolink^?PLA^?MINUS oligonucleotide. This antibody detects tyrosine phosphorylated proteins in all species. Protein target specificity can be obtained by pairing with an antibody specific to a protein of interest in a Duolink PLA experiment. This product must be used in combination with a PLUS PLA probe of a different species or another directly-conjugated primary antibody with a PLUS PLA oligo. The Anti-mouse PLA probe MINUS (DUO92001) is not necessary when using this product.

特异性

This directly-conjugated primary antibody PLA probe recognizes tyrosine-phosphorylated proteins from all species

应用

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink^?In SituFluorescence Protocol and the Duolink^?In SituBrightfield Protocol depending on the detection reagents used.

Visit our Duolink^?PLA Resource Center for information on how to run a Duolink^?experiment, applications, troubleshooting, and more.

To perform a complete Duolink^?PLAin situexperiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^?PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Application Note
Protein phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Anti-phosTyr antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosine phosphorylation of the protein of interest. This process is time-consuming and cannot provide subcellular localization.

Anti-phosTyr PLA probes are used to detect the phosphorylation of tyrosine residues in proteins. Protein target specificity can be obtained by pairing with an antibody specific to a protein of interest in a Duolink^?PLA^?experiment. This product is particularly useful when site-specific anti-phosphorylation antibodies are not available. A PLUS PLA probe of a different species or another directly-conjugated primary antibody with a PLUS PLA oligo must be used in combination with this product. The Anti-mouse PLA probe MINUS (DUO92001) is not necessary when using this product. See our Product Selection Guide for more information.

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特点和优势

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

法律信息

4G10 is a registered trademark of Upstate Group, Inc.
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified antibody (Protein G-Sepharose)
antibody product type	primary antibodies
克隆	4G10?, monoclonal
半岛bd体育手机客户端 线	Duolink?
species reactivity	all
technique(s)	proximity ligation assay: suitable
适用性	suitable for brightfield suitable for fluorescence
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable