Duolink^?PLA Probe Goat IgG Isotype Control MINUS from goat

半岛bd体育手机客户端 说明

一般描述

An immunoglobulin G (IgG) isotype control antibody with the Duolink PLA MINUS oligonucleotide specific for goat IgG. This is a convenience product to be used in place of a goat primary antibody to determine non-specific background signal and optimal antibody dilution. The anti-goat PLA probe MINUS is not necessary when using this product, but must be used in combination with a PLUS PLA probe.

应用

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink^?In SituFluorescence Protocol and the Duolink^?In SituBrightfield Protocol depending on the detection reagents used.

Visit our Duolink^?PLA Resource Center for information on how to run a Duolink^?experiment, applications, troubleshooting, and more.

Application Note
To perform a complete Duolink^?PLA in situexperiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^?PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Isotype control PLA probes are used in lieu of a primary antibody in a Duolink^?PLAin situexperiment to determine background signal contributed by non-specific binding of the primary antibody. Isotype control PLA probes can also be used to determine the optimal titer conditions of primary antibody PLA probes.

A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

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特点和优势

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
生物来源	goat (polyclonal)
抗体形式	affinity purified immunoglobulin (Isotype control antibody)
半岛bd体育手机客户端 线	Duolink?
technique(s)	proximity ligation assay: suitable
适用性	suitable for brightfield suitable for fluorescence
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable