半岛bd体育手机客户端 说明
应用
Specificity
Fluorescence detection reagents are often used with FITC, Cy?3,Texas Red?or Cy?5 filter. The filter chosen is dependent on the color of the Multicolor Probemaker kit selected.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink?in situreagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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特点和优势
Multicolor Duolink?proximity ligation assay (PLA?) is an application of the Duolink?technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Multicolor Duolink PLA protocol to use this product.
法律信息
Cy is a registered trademark of Cytiva
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC
Texas Red is a registered trademark of Life Technologies
半岛bd体育手机客户端 性质
半岛bd体育手机客户端 线 | Duolink? |
technique(s) | immunofluorescence: suitable multiplexing: suitable proximity ligation assay: suitable |
适用性 | suitable for fluorescence |
运输 | dry ice |
储存温度 | ?20℃ |
Sigma-Aldrich