DNMT1 Active human

半岛bd体育手机客户端 说明

一般描述

DNMT1 (DNA methyltransferase 1) is a member of the DNMT enzyme family which also includes DNMT2, DNMT3A, and DNMT3B. It is a multidomain protein composed of 1616 amino acids. It has a methyltransferase domain in its C-terminal, which shows sequence homology to bacterial methyltransferases.
The DNMT1 gene is mapped to human chromosome 19p13.2.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. DNMT1 Active human has been used in RNA electrophoretic mobility shift assay.
DNMT1 (DNA methyltransferase 1) has been used for RNA electrophoretic mobility shift assay (REMSA) to determine the role of extra-coding RNAs (ecRNAs) in controlling neuronal DNA methylation, through interaction with DNA methyltransferases.

生化/生理作用

DNMT1 (DNA methyltransferase 1) is the predominant enzyme that is responsible for maintaining the level of DNA methylation. It is responsible for replicating CpG methylation patterns from parent to daughter DNA strands, thus, creating heritable methylation signatures through cell division. It, therefore, maintains epigenetic state of DNA. Abnormalities in the expression of this protein are linked with the progression and prognosis of multiple cancers, such as hepatocellular carcinoma, pancreatic, lung and bladder cancers. Up-regulation of this protein in GC (gastric cancer) with less differentiation, advanced stage and increased rate of mortality. Upregulation of the DNMT1 gene indicates poor prognosis in malignant cancers including renal cell carcinoma, lymphoma, pancreatic and and bladder cancer.

单位定义

One unit is defined as the amount of enzyme required to methylate 1 pmol of DNA substrate/min at 37℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in baculovirus infected insect cells
标签	GST tagged (N-terminal)
测定	≥50% (SDS-PAGE)
形式	aqueous solution
效能	≥50
分子量	211 kDa
包装	pkg of 10 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	0.12 mg/mL
NCBI登记号	NM_001130823
UniProt登记号	P26358
运输	dry ice
储存温度	?70℃
Gene Information	human ... DNMT1(1786)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable