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位置: 首页> 品牌> Sigma-Aldrich> 2-(4-氨基苯基)-6-吲哚甲酰胺 二盐酸盐

DAPI ready made solution with Antifade

4',6-二脒基-2-苯基吲哚 二盐酸盐

品牌
Sigma-Aldrich
CAS
28718-90-3
货号
MBD0020
规格纯度
For Nuclear counterstain in Fluorescence Microscopy
价格
1325.03 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
28718-90-3
DAPI ready made solution with Antifade
2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
DAPI Stain
DAPI Dye
DNA Content Counterstain
4′,6-Diamidino-2-phenylindole dihydrochloride
DAPI dihydrochloride
4',6-二脒基-2-苯基吲哚 二盐酸盐
DAPI 二盐酸盐
2-(4-氨基苯基)-6-吲哚甲酰胺 二盐酸盐
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Most of the fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. DAPI Ready Made Solution with Antifade is a formulation that suppress photobleaching and prolong fluorescent signals.

应用

DAPI Ready Made Solution with Antifade is a glycerol based ready to use solution provided at 1μg/mL concentration in a dropper vial that can be applied directly to cells or tissue samples on microscope slides or coverslips. It is recommended to warm up at room temperature and mix by swirling the dropper vial before use. Store DAPI Ready Made Solution with antifade at -20 C protected from light.
在琼脂糖凝胶中染色DNA,DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱,通过对双链复合体的特殊可视化检测印迹应用中的退火探针,并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

生化/生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟(富含AT的DNA优先)结合,形成稳定的复合物,其发出的荧光比单独的DAPI高约20倍。

特点和优势

  • Protects dyes from fading during imaging.
  • Ready-to-use liquid.
  • Easy to use Dropper vial application.
  • Stained sample are stable for longer time period.
  • Maintains fluorescence signal-little to no quenching.

外形

Liquid - DAPI readymade with antifade solution is provided at 1μg/mL concentration in glycerol-PBS based solution.

基本信息

经验(实验)分子式 C16H15N5· 2HCl
分子量 350.25
Beilstein 4894417
MDL编号 MFCD00012681

半岛bd体育手机客户端 性质

形式 liquid
储存条件 protect from light
technique(s) transfection: suitable
荧光 λex340 nm; λem488 nm (nur DAPI)
λex364 nm; λem454 nm (DAPI-DNA-Komplex)
运输 dry ice
储存温度 ?20℃
SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N

安全信息

危险声明 H412
预防措施声明 P273 - P501
危险分类 Aquatic Chronic 3
储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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