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Chitinase fromStreptomyces griseus

灰链霉菌来源几丁质酶

品牌
Sigma-Aldrich
货号
SAE0158
规格纯度
chromatographically purified, lyophilized powder, free of DNA contaminants, suitable for Microbiome research
价格
2138.29 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Chitinase from Streptomyces griseus
Chitodextrinase
(1→4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase)
Poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase
Lytic Enzyme
Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase
beta-1,4-poly-N-acetyl glucosamidinase
Hydrolitic enzyme
poly-beta-glucosaminidase
灰链霉菌 来源几丁质酶
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeletal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates). Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.

The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:

  • The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
  • The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.

应用

  • Agriculture fields: control pathogens.
  • Human health care: Asthma.
  • Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
  • Preparation of single-cell protein
  • Isolation of protoplasts from fungi and yeast
  • Control of pathogenic fungi
  • Treatment of chitinous waste, mosquito control and morphogenesis
The study of microbial communities has recently been revolutionized by the widespread adoption of culture- independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. Purified Chitinase SAE0158 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA, using 35 cycles of PCR amplification of 16S and 18S rDNA, using universal primer sets.

特点和优势

This Chitinase is free of DNA contaminates, suitable for microbiome research. Cell walls digestion is performed safely by chitin degradation in fungi and other organisms.

单位定义

One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 ℃ in a 2-hour assay.

半岛bd体育手机客户端 性质

质量水平 300
specific activity >200 USP units/mg solid
特点 DNA free
运输 wet ice
储存温度 ?20℃

安全信息

象形图 GHS08
警示用语: Danger
危险声明 H334
预防措施声明 P261 - P284 - P501
危险分类 Resp. Sens. 1
储存分类代码 11 - Combustible Solids
WGK WGK 3

Sigma-Aldrich

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