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ChemiSCREEN MC3 Membrane Preparation

品牌
Sigma-Aldrich
货号
HTS022M
包装型号
规格纯度
价格
10463.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
ChemiSCREEN MC3 Membrane Preparation
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Human MC3
The melanocortins, the peptides alpha-, beta- and gamma-melanocyte-stimulating hormone (MSH), bind to a family of five Gs-coupled GPCR receptors (MCRs) and play important roles in energy balance, reproductive function, pigmentation and inflammation (Gantz and Fong, 2003). MC3R is expressed in the CNS, including the hypothalamus, and appears to play a role in energy homeostasis as indicated by the observation that MC3R-null mice have increased subcutaneous fat, a loss of lean body mass, and reduced energy expenditure (Butler et al., 2000; Chen et al., 2000). Macrophages also express MC3R, which together with MC1R mediates the anti-inflammatory activity of melanocortins in animal models of peritonitis and myocardial infarct (Getting et al., 2003; 2004). Chemicon′s MC3R membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of MC3R interactions with melanocortins. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-MSH. With 10.0 mg/well MC3R Membrane Prep and 0.25 nM [125I]-MSH, a greater than 7-fold signal-to-background ratio is obtained.

应用

Radioligand binding assay, and GTPgammaS binding.
Human MC3 GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.

生化/生理作用

  • GPCR Class: A
  • Protein Target: MC3
  • Target Sub-Family: Melanocortin

半岛bd体育手机客户端 规格

Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 9 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.Binding buffer: 25 mM Hepes, pH 7.0, 1 mM MgCl2, 1.5 mM CaCl22H2O, 100mM NaCl, 0.2% BSA, Protease Inhibitor, filter and stored at 4 ℃.

Radioligand: [125I] MSH (Perkin Elmer# NEX352)

Wash Buffer: 25 mM Hepes, pH 7.0, 1 mM MgCl2, 1.5 mM CaCl22H2O, 100mM NaCl, 0.2% BSA, filtered and stored at 4℃. One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled MSH at 0.25 nM

外形

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80℃.

储存及稳定性

Maintain frozen at -70℃ for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

基本信息

eCl@ss 32161000

半岛bd体育手机客户端 性质

质量水平 100
生物来源 human
重组 expressed in Chem-1 cells
manufacturer/tradename ChemiScreen
Chemicon?
technique(s) ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBI登记号 NM_019888.2
UniProt登记号 P41968

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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